CWD TRANSMITS TO MACAQUE BY ORAL INTAKE MUSCLE

Discussion in 'Whitetail Deer Management' started by flounder9, Jun 5, 2017.

  1. Notice to Members Regarding Chronic Wasting Disease (CWD)
    Posted on: May 31st, 2017
    To: MNA Members
    From: Métis Nation of Alberta
    Date:
    Wednesday, May 31, 2017
    Métis Nation of Alberta (MNA) was made aware of a recent Canadian research study examining the transmission of Chronic Wasting Disease. The initial results of the study indicate that macaque monkeys (genetically similar to humans) can be infected with Chronic Wasting Disease (CWD) after eating deer that is infected with CWD. CWD is a prion disease, which are fatal, transmissible diseases characterized by abnormal proteins in the brain and nervous system. To date no research has shown that CWD can be passed on to humans, and no human cases of CWD have ever been identified. However, this new research indicates that it is a possibility. The Deputy Chief Medical Officer of Health has reached out to us to share with our Métis harvesters this important information.
    For more information you can visit:

    http://aep.alberta.ca/fish-wildlife...onic-wasting-disease/cwd-updates/default.aspx

    and

    www.nwhc.usgs.gov/disease_information/chronic_wasting_disease/index.jsp.

    What the Alberta Government knows:
    • CWD is present in southeastern Alberta, with the area slowly spreading westward over time (introduced into Alberta from Saskatchewan) – see map for more information at http://aep.alberta.ca/fish-wildlife...uments/HuntersCWD-HarvestedDeerHeads-2016.pdf
    • CWD circulates in deer populations, particularly mule deer; it has been found in about 4% of deer tested in 2016;
    • Elk can be infected in areas where CWD has been present in deer for a long period of time;
    • Moose can also be infected, but this would be fairly rare.
    Necessary Precautions for Harvesters:
    1. Hunters and others who handle carcasses follow basic handling precautions (available here http://aep.alberta.ca/fish-wildlife...DeerCarcassTransportationHandling-Oct2009.pdf
    2. All deer, moose and elk harvested from CWD mandatory submission wildlife management units (WMUs) be tested for CWD; and
    3. A negative result (no CWD detected by the test) must be obtained before any part of an animal is eaten.

    For more information, contact:
    Amy Quintal
    Métis Nation of Alberta
    Métis Harvesting Liaison
    Tel: (780) 455 – 2200
    aquintal@metis.org

    http://albertametis.com/2017/05/notice-members-regarding-chronic-wasting-disease-cwd/

    FRIDAY, JUNE 02, 2017
    Alberta Canada Chronic Wasting Disease (CWD) Surveillance Update: 2016/17 Final
    http://chronic-wasting-disease.blogspot.com/2017/06/alberta-canada-chronic-wasting-disease.html

    Chronic Wasting Disease: CFIA Research Summary
    Embargoed until May 23, 2017
    (OCR of a scanned original)
    Research Findings
    Chronic Wasting Disease (CWD) is a progressive, fatal disease of the nervous system of cervids including deer, elk, moose, and reindeer that is caused by abnormal proteins called prions. It is known as a transmissible spongiform encephalopathy (TSE). Other TSEs include scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle, and Creutzfeldt-Jakob disease (CJD) in humans.
    A limited number of experimental studies have demonstrated that non-human primates, specifically squirrel monkeys, are susceptible to CWD prions. An ongoing research study has now shown that CWD can also be transmitted to macaques, which are genetically closer to humans.
    The study led by Dr. Stefanie Czub, a scientist at the Canadian Food Inspection Agency (CFIA), and funded by the Alberta Prion Research institute has demonstrated that by orally administering material under experimental conditions from cervids (deer and elk) naturally infected with CWD, the disease can be transmitted to macaques.
    in this project, which began in 2009, 18 macaques were exposed to CWD in a variety of ways: by injecting into the brain, through contact with skin, oral administration, and intravenously (into the bloodstream through veins). So far, results are available from 5 animals. At this point, two animals that were exposed to CWD by direct introduction into the brain, one that was administered infected brain material by oral administration and two that were given infected muscle by oral administration have become infected with CWD. The study is ongoing and testing continues in the remaining animals. The early results will be presented at PRlON 2017, the annual international conference on prion diseases, in Edinburgh, Scotland, May 23 to 26, 2017.
    Potential impacts of the new finding
    Since 2003 Canada has a policy that recommends that animals and materials known to be infected with prions be removed from the food chain and from health products. Although no direct evidence of CWD prion transmission to humans has ever been recorded, the policy advocates a precautionary approach to managing CWD and potential human exposure to prions. These initial findings do not change Health Canada’s Health Products and Food Branch (HPFB) position on food and health products. A precautionary approach is still recommended to manage the potential risks of exposure to prions through food and health products. Measures are in place at federal, provincial and territorial levels to reduce human exposure to products potentially contaminated by CWD by preventing known infected animals from entering the marketplace.
    While Federal and P/T government’s animal disease control policies continue to divert known CWD-infected animals away from entering the food and feed supply, research and development of sensitive detection methods including live-animal sampling techniques remain crucial for ensuring an accurate diagnosis. In addition, consistent federal, provincial and territorial communications of appropriate precautionary measures for hunters and indigenous communities are required.
    Next Steps
    The CFlA will continue to collaborate with national and international partners to develop and validate new diagnostic techniques. The CFlA will also continue to offer confirmatory testing services and reference laboratory expertise to provincial and territorial partners on demand.
    Currently, CFlA laboratories are leading or collaborating on several research projects to understand the potential for CWD to infect humans. These projects use non‐human primates, genetically modified mice, and cell-free amplification approaches. Given the present findings, CFiA encourages continued research into TSEs.
    The results of this study reinforce the need to redesign the federal program to foster greater adoption of risk mitigation measures for farmed cervids. Federal and provincial government collaboration will continue as new program options are assessed.
    The results of Dr. Czub’s research into CWD will be of interest to scientists, governments, industry and people who consume cervid products. After the presentation at PRION 2017, the research will follow the normal steps of completion, peer review and publication. The Government of Canada will monitor the response to this research and determine whether further review of the science is required. Other studies underway by other researchers may also become public as a result of the presentation of Dr. Czub’s research.
    The Public Health Agency of Canada, Health Canada, CFlA and other Federal partners are working together to assess what policies or programs need further review as well as preparing other communications about the research and health policy and advice to Canadian. 2017/04/28
    ===end...UNOFFICIAL...NO URL LINK...TSS===
    0:30 First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques: a work in progress
    Dr Stefanie Czub University of Calgary Faculty of Veterinary Medicine/Canadian Food Inspection Agency Canada
    http://prion2017.org/programme/

    WEDNESDAY, MAY 03, 2017
    *** First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques
    http://chronic-wasting-disease.blogspot.com/2017/05/first-evidence-of-intracranial-and.html

    Wednesday, May 24, 2017

    PRION2017 CONFERENCE VIDEO UPDATE 23 – 26 May 2017 Edinburgh UPDATE 1

    Subject: PRION2017 CONFERENCE VIDEO UPDATE 23 – 26 May 2017 Edinburgh

    *see archives of previous Prion Conferences, the ones that are still available, scroll down towards bottom in this link.

    http://prionprp.blogspot.com/2017/05/prion2017-conference-video-update-23-26.html
    WEDNESDAY, MAY 31, 2017
    Texas New Exotic CWD Susceptible Species Rules Now in Effect
    http://chronic-wasting-disease.blogspot.com/2017/05/texas-new-exotic-cwd-susceptible.html
     
  2. Couple years ago I killed a buck that had the Hershey squirts running down both back legs. After asking alot of questions to different people it was determined I should be able to eat it and we did. I will say I was very hesitant about it regardless of what studies say. He was acting normal working scrapes and had a ton of corn in his gut which is extremely rare where I hunt since it's all hardwoods so that helped with the decision to eat him.
     

  3. The elephant in the room...

    The results of this study reinforce the need to redesign the federal program to foster greater adoption of risk mitigation measures for farmed cervids.
     
    bluedog and Deehntr56 like this.
  4. One elephant looks like a deer feeder.
    Many elephants are out and about and....routinely ignored.
     
    UNCLEMIKE likes this.
  5. Steve

    Steve Staff Member Admin Mod

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  6. Subject: PRION 2017 CONFERENCE ABSTRACT Chronic Wasting Disease in European moose is associated with PrPSc features different from North American CWD

    P178 Chronic Wasting Disease in European moose is associated with PrPSc features different from North American CWD

    Dr Laura Pirisinu1, Dr Linh Tran2, Dr Gordon Mitchell3, Dr Aru Balachandran3, Dr Thierry Baron4, Dr Cristina Casalone5, Dr Michele Di Bari1, Dr Umberto Agrimi1. Dr Romolo Nonno1, Dr Sylvie Benestad2

    1Department of Veterinary Public Health and Food Safety, Istituto Superiore di Sanita, Rome, Italy, 2Norwegian Veterinary Institute, Oslo, Norway, 3Canadian Food Inspection Agency, National and OlE Reference Laboratory for Scrapie and CWD, Ottawa Laboratory Fallowfield, Ottawa, Canada, 4Neurodeqenerative Diseases Unit, ANSES - French Agency for Food, Environmental and Occupational Health & Safety, Lyon, France, 5Istituto Zooprofilattico Sperimentale del Pietnonte, Liguria e Valle d'Aosta, Turin, Italy

    Aims: In 2016, Chronic Wasting Disease (CWD) was detected for the first time in Europe in three wild Norwegian reindeer (Rangifer tarandus tarandus) and in two moose (Alces alces). The biochemical analysis and the immunohistochemical distribution of PrPSc from Norwegian reindeer revealed a pattern similar to North American (NA) isolates1. In this study, we studied the biochemical features of PrPSc from the two CWD cases in Norwegian moose.

    Methods: Western blot (WB) analysis of PK-treated PrPSc (PrPres) from Norwegian moose and reindeer isolates was performed according to the ISS discriminatory WB protocol (used in BSE and scrapie Italian surveillance). PrPres fragments were determined by epitope mapping (SAF84, L42, 9A2, 12B2 mAbs), before and after deglycosylation. CWD isolates from Canadian cervids (wapiti, moose and white tailed deer) and a panel of small ruminant and bovine prion strains circulating in Europe were also analysed.

    Results: WB analysis with different mAbs showed that PrPres from both Norwegian moose samples was different from that usually associated with CWD in cervids. Indeed, their main C-terminal fragment had a MW lower than the other CWD isolates, and could be discriminated by the absence of the 12B2 epitope. Furthermore, while NA CWD PrPSc is composed of a single PrPres fragment, Norwegian moose samples had an additional C-terminal PrPres fragment of ~13 kDa (CTF13).
    Among ovine TSEs, classical scrapie and Nor98 were discriminated from both Norwegian moose isolates, while CH1641 samples had molecular features partially overlapping with the moose, i.e. a low MW PrPres and the presence of CTF13. In contrast, moose PrPSc did not overlap with any bovine PrPSc. Indeed, the MW of moose PrPres was lower than H-BSE and similar to C-BSE and L-BSE PrPres, but the two bovine prions lacked additional PrPres fragments.

    Conclusions: Unexpectedly, PrPSc from Norwegian moose revealed features substantially different from all other CWD isolates. The PrPSc pattern of Norwegian moose was also different from Canadian moose, suggesting that the variant PrPSc type observed does not simply reflect a host factor and could represent a new CWD strain. Furthermore, PrPSc of Norwegian moose can be easily discriminated from all BSE types, classical scrapie and Nor98, while showing significant overlapping only with CH1641.
    Bioassay in voles will help to clarify whether the different PrPSc types observed reflect the presence of a new CWD strain in Norwegian moose, and its relationships with known animal TSEs.

    References: 1Benestad et al, Vet Res (2016}47:88

    PRION 2017 DECIPHERING NEURODEGENERATIVE DISORDERS

    please see;

    ***Our transmission study demonstrates that CH 1641-like scrapie is likely to be more virulent than classical scrapie in cattle.

    In the US, scrapie is reported primarily in sheep homozygous for 136A/171Q (AAQQ) and the disease phenotype is similar to that seen with experimental strain CH1641.

    http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=182469

    ***Our transmission study demonstrates that CH 1641-like scrapie is likely to be more virulent than classical scrapie in cattle.

    P-088 Transmission of experimental CH1641-like scrapie to bovine PrP overexpression mice

    Kohtaro Miyazawa1, Kentaro Masujin1, Hiroyuki Okada1, Yuichi Matsuura1, Takashi Yokoyama2

    1Influenza and Prion Disease Research Center, National Institute of Animal Health, NARO, Japan; 2Department of Planning and General Administration, National Institute of Animal Health, NARO

    Introduction: Scrapie is a prion disease in sheep and goats. CH1641-lke scrapie is characterized by a lower molecular mass of the unglycosylated form of abnormal prion protein (PrpSc) compared to that of classical scrapie. It is worthy of attention because of the biochemical similarities of the Prpsc from CH1641-like and BSE affected sheep. We have reported that experimental CH1641-like scrapie is transmissible to bovine PrP overexpression (TgBoPrP) mice (Yokoyama et al. 2010). We report here the further details of this transmission study and compare the biological and biochemical properties to those of classical scrapie affected TgBoPrP mice.

    Methods: The details of sheep brain homogenates used in this study are described in our previous report (Yokoyama et al. 2010). TgBoPrP mice were intracerebrally inoculated with a 10% brain homogenate of each scrapie strain. The brains of mice were subjected to histopathological and biochemical analyses.

    Results: Prpsc banding pattern of CH1641-like scrapie affected TgBoPrP mice was similar to that of classical scrapie affected mice. Mean survival period of CH1641-like scrapie affected TgBoPrP mice was 170 days at the 3rd passage and it was significantly shorter than that of classical scrapie affected mice (439 days). Lesion profiles and Prpsc distributions in the brains also differed between CH1641-like and classical scrapie affected mice.

    Conclusion: We succeeded in stable transmission of CH1641-like scrapie to TgBoPrP mice. Our transmission study demonstrates that CH 1641-like scrapie is likely to be more virulent than classical scrapie in cattle.
    snip...
    In the US, scrapie is reported primarily in sheep homozygous for 136A/171Q (AAQQ) and the disease phenotype is similar to that seen with experimental strain CH1641.

    http://www.ars.usda.gov/research/publications/publications.htm?seq_no_115=182469

    snip...see ;

    TUESDAY, JUNE 13, 2017

    PRION 2017 CONFERENCE ABSTRACT Chronic Wasting Disease in European moose is associated with PrPSc features different from North American CWD

    http://chronic-wasting-disease.blogspot.com/2017/06/prion-2017-conference-abstract-chronic.html

    WEDNESDAY, MAY 03, 2017 (UPDATED)

    *** First evidence of intracranial and peroral transmission of Chronic Wasting Disease (CWD) into Cynomolgus macaques

    http://chronic-wasting-disease.blogspot.com/2017/05/first-evidence-of-intracranial-and.html


    MONDAY, JUNE 12, 2017

    Rethinking Major grain organizations opposition to CFIA's control zone approach to Chronic Wasting CWD TSE Prion Mad Deer Type Disease 2017?

    http://chronic-wasting-disease.blogspot.com/2017/06/rethinking-major-grain-organizations.html

    TUESDAY, JUNE 20, 2017

    Norway Confirms 6th Case of Skrantesjuke CWD TSE Prion Disease

    http://chronic-wasting-disease.blogspot.com/2017/06/norway-confirms-6th-case-of.html